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Human Solute Carrier Family 16,Member 3 (SLC16A3) ELISA Kit

SKU:
HUDL02698
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O15427
Sensitivity:
0.28ng/mL
Range:
0.78-50ng/mL
ELISA Type:
Sandwich
Synonyms:
MCT4,Monocarboxylic Acid Transporter 4,Monocarboxylate Transporter 4
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

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Human Solute Carrier Family 16,Member 3 (SLC16A3) ELISA Kit

The Human Solute Carrier Family 16 Member 3 (SLC16A3) ELISA Kit is specifically designed for the accurate detection of SLC16A3 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reliable results for a variety of research applications.SLC16A3, also known as monocarboxylate transporter 4 (MCT4), plays a critical role in the transport of monocarboxylates across cell membranes, including lactate and pyruvate. It is involved in various physiological processes, such as energy metabolism and pH regulation, making it a key target for studying metabolic disorders, cancer metabolism, and other related conditions.

With this ELISA kit, researchers can efficiently quantify SLC16A3 levels in biological samples, allowing for a better understanding of its function and potential implications in disease pathology. It is a valuable tool for scientists working in the fields of metabolism, oncology, and molecular biology.

Product Name:Human Solute Carrier Family 16,Member 3 (SLC16A3) ELISA Kit
Product Code:HUDL02698
Size:96 Assays
Target:SLC16A3
Synonyms:MCT4, Monocarboxylic Acid Transporter 4, Monocarboxylate Transporter 4
Detection Method:Sandwich
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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