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Human Solute Carrier Family 16,Member 1 (SLC16A1) ELISA Kit

SKU:
HUDL02697
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P53985
Sensitivity:
0.116ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
MCT1,MCT,Monocarboxylate Transporter 1,Monocarboxylic Acid Transporter 1
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Solute Carrier Family 16,Member 1 (SLC16A1) ELISA Kit

The Human SLC16A1 ELISA Kit is specifically designed for the precise measurement of solute carrier family 16 member 1 (SLC16A1) levels in human samples including serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, ensuring accurate and reproducible results for various research applications.SLC16A1 is a vital transporter protein involved in the transportation of monocarboxylates across cell membranes, playing a crucial role in metabolism and energy production.

Dysregulation of SLC16A1 has been associated with metabolic disorders, cancer, and neurological diseases, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.Overall, the Human SLC16A1 ELISA Kit provides researchers with a powerful tool for investigating the role of SLC16A1 in health and disease, offering insight into potential diagnostic and therapeutic strategies.

Product Name:Human Solute Carrier Family 16,Member 1 (SLC16A1) ELISA Kit
Product Code:HUDL02697
Size:96 Assays
Target:SLC16A1
Synonyms:MCT1, MCT, Monocarboxylate Transporter 1, Monocarboxylic Acid Transporter 1
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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