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Human Solute Carrier Family 1, Member 5 (SLC1A5) ELISA Kit

SKU:
HUDL02699
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q15758
Sensitivity:
0.112ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
SLC1A5,AAAT,ASCT2,ATBO,M7V1,M7VS1,R16,RDRC,Neutral Amino Acid Transporter B(0),Baboon M7 virus receptor,Sodium-dependent neutral amino acid transporter type 2
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Solute Carrier Family 1, Member 5 (SLC1A5) ELISA Kit

The Human SLC1A5 ELISA Kit is specifically designed to detect levels of solute carrier family 1 member 5 (SLC1A5) in human samples including serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, ensuring accurate and reproducible results for a variety of research applications.SLC1A5 is an important protein involved in amino acid transport, particularly the uptake of glutamine, which is essential for cell growth and proliferation.

Dysregulation of SLC1A5 has been associated with various diseases including cancer, making it a valuable biomarker for studying disease progression and potential therapeutic interventions.By using the Human SLC1A5 ELISA Kit, researchers can gain valuable insights into the role of SLC1A5 in health and disease, ultimately leading to a better understanding of its implications in various pathological conditions.

Product Name:Human Solute Carrier Family 1, Member 5 (SLC1A5) ELISA Kit
Product Code:HUDL02699
Size:96 Assays
Target:SLC1A5
Synonyms:SLC1A5, AAAT, ASCT2, ATBO, M7V1, M7VS1, R16, RDRC, Neutral Amino Acid Transporter B(0), Baboon M7 virus receptor, Sodium-dependent neutral amino acid transporter type 2
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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