Human Sodium/Hydrogen Exchanger 1 (NHE1) ELISA Kit (HUDL02035)
SKU:
HUDL02035
UPC:
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P19634
Sensitivity:
0.064ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
SLC9A1,APNH,Solute Carrier Family 9 Member 1,Sodium/Hydrogen Exchanger,Antiporter,Na+/H+,Amiloride Sensitive,Na(+)/H(+) antiporter, amiloride-sensitive
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
Human Sodium/Hydrogen Exchanger 1 (NHE1) ELISA Kit
The Human Sodium Hydrogen Exchanger-1 (NHE1) ELISA Kit is specifically designed for the accurate quantification of NHE1 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for various research applications.NHE1 is a key regulator of intracellular pH and cell volume, playing a crucial role in maintaining cellular homeostasis. Dysregulation of NHE1 has been implicated in various diseases such as cancer, cardiovascular disorders, and neurological conditions.
As such, measuring NHE1 levels can provide valuable insights into disease pathogenesis and potential therapeutic interventions.With its advanced technology and reliability, the Human NHE1 ELISA Kit is an indispensable tool for researchers studying the molecular mechanisms underlying disease progression and exploring new treatment strategies. Get your kit today and unlock the potential of NHE1 research.
Product Name:
Human Sodium/Hydrogen Exchanger 1 (NHE1) ELISA Kit
Product Code:
HUDL02035
Size:
96 Assays
Target:
NHE1
Synonyms:
SLC9A1, APNH, Solute Carrier Family 9 Member 1,Sodium/Hydrogen Exchanger, Antiporter,Na+/H+,Amiloride Sensitive, Na(+)/H(+) antiporter, amiloride-sensitive
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
