Human SOD3 (Superoxide Dismutase 3, Extracellular) ELISA Kit
The Human SOD3 (Superoxide Dismutase 3) Extracellular ELISA Kit is specially designed for the quantitative detection of SOD3 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for various research purposes.SOD3, also known as extracellular superoxide dismutase, plays a vital role in antioxidant defense by catalyzing the dismutation of superoxide radicals. Dysregulation of SOD3 has been linked to oxidative stress-related diseases such as cardiovascular disorders, neurodegenerative conditions, and inflammatory diseases.
With the Human SOD3 Extracellular ELISA Kit, researchers can study the role of SOD3 in oxidative stress-related pathologies and potentially develop therapeutic interventions targeting antioxidant pathways. Trust in the precision and reliability of this ELISA kit for confident and insightful research findings.
Product Name:
Human SOD3 (Superoxide Dismutase 3, Extracellular) ELISA Kit
SKU:
HUES03252
Target:
Human SOD3 (Superoxide Dismutase 3, Extracellular)
Size:
96T
Assay type:
Competitive-ELISA
Assay time:
2.0h
Sensitivity:
3.75 ng/mL
Detection range:
6.25-400 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with SOD3. During the reaction, SOD3 in the sample or standard competes with a fixed amount of SOD3 on the solid phase supporter for sites on the Biotinylated Detection Ab specific to SOD3. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of SOD3 in tested samples can be calculated by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-112
90-101
86-101
Average (%)
105
96
93
1:4
Range (%)
89-101
87-100
101-113
Average (%)
94
94
107
1:8
Range (%)
88-98
87-99
96-107
Average (%)
93
94
102
1:16
Range (%)
88-104
91-105
97-108
Average (%)
95
97
102
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-108
99
EDTA plasma (n=5)
87-100
93
Cell culture media (n=5)
91-104
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
20.3
63.4
182.7
22.1
66.3
166.5
Standard deviation
1.3
2.7
5.7
1.2
3.0
7.2
C V (%)
6.4
4.26
3.12
5.43
4.52
4.32
Sample type &Sample volume:
serum, plasma and other biological fluids; 50μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of SOD3 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes SOD3 in samples. No significant cross-reactivity or interference between SOD3 and analogues was observed.
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