Human SOD2 (Superoxide Dismutase 2, Mitochondrial) ELISA Kit
The Human SOD2 (Superoxide Dismutase 2, Mitochondrial) ELISA Kit is a specialized assay designed for the precise and quantitative detection of human SOD2 levels in various biological samples. Superoxide Dismutase 2 (SOD2) is a critical mitochondrial antioxidant enzyme that plays a pivotal role in protecting cells against oxidative stress by scavenging harmful superoxide radicals. Mitochondrial SOD2 is essential for maintaining cellular homeostasis, redox balance, and protecting vital organelles from oxidative damage. This ELISA kit offers researchers a reliable means to quantify SOD2 expression levels, providing valuable insights into the antioxidant defense mechanisms within mitochondria.
By accurately measuring SOD2 levels, researchers can study oxidative stress-related conditions, mitochondrial dysfunction, and diseases associated with impaired antioxidant responses. The Human SOD2 ELISA Kit is characterized by exceptional sensitivity and specificity, ensuring accurate and reproducible results. Manufactured under stringent quality control standards, this kit delivers robust performance and user-friendly protocols, making it an optimal choice for investigations focused on mitochondrial antioxidant systems, oxidative stress, and related pathophysiological processes in human samples.
Product Name:
Human SOD2 (Superoxide Dismutase 2, Mitochondrial) ELISA Kit
SKU:
AEES00654
Target:
Human SOD2 (Superoxide Dismutase 2, Mitochondrial)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SOD2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SOD2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SOD2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SOD2. You can calculate the concentration of Human SOD2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-103
87-100
94-106
Average (%)
97
93
101
1:4
Range (%)
88-100
83-97
94-111
Average (%)
94
90
102
1:8
Range (%)
90-106
85-97
95-108
Average (%)
98
91
102
1:16
Range (%)
93-106
83-95
92-107
Average (%)
99
89
98
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-107
98
EDTA plasma (n=5)
89-101
95
Cell culture media (n=5)
92-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.93
1.79
9.97
0.89
1.68
10.95
Standard deviation
0.06
0.1
0.51
0.06
0.08
0.56
C V (%)
6.25
5.68
5.15
6.84
5.04
5.09
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SOD2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SOD2 in samples. No significant cross-reactivity or interference between Human SOD2 and analogues was observed.
