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Human Slit Homolog 1 (Slit1) ELISA Kit

SKU:
HUDL02712
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O75093
Sensitivity:
0.064ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
MEGF4,SLIL1,SLIT3,Multiple epidermal growth factor-like domains protein 4
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Slit Homolog 1 (Slit1) ELISA Kit

The Human SLIT Homolog 1 (SLIT1) ELISA Kit is specifically designed for the accurate measurement of SLIT1 levels in human serum, plasma, and cell culture supernatants. This kit is highly sensitive and specific, ensuring reliable and reproducible results for a variety of research applications.SLIT1 is a critical protein involved in axon guidance and cell migration, playing a key role in the development of the nervous system. Dysregulation of SLIT1 has been linked to neurological disorders such as autism and schizophrenia, making it an important biomarker for studying these conditions and potentially developing new therapeutic approaches.

Overall, the Human SLIT Homolog 1 (SLIT1) ELISA Kit provides researchers with a powerful tool for accurately measuring SLIT1 levels and gaining valuable insights into its role in various physiological and pathological processes.

Product Name:Human Slit Homolog 1 (Slit1) ELISA Kit
Product Code:HUDL02712
Size:96 Assays
Target:Slit1
Synonyms:MEGF4, SLIL1, SLIT3, Multiple epidermal growth factor-like domains protein 4
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)82-10292
EDTA plasma (n=5)91-9894
Heparin plasma (n=5)86-10193
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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