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Human Secondary Lymphoid Tissue Chemokine (SLC) ELISA Kit

SKU:
HUDL02711
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O00585
Sensitivity:
12.3pg/mL
Range:
31.25-2000pg/mL
ELISA Type:
Sandwich
Synonyms:
CCL21,6Ckine,CKb9,ECL,SCYA21,TCA4,ECL,Chemokine(C-C-Motif)Ligand 21,Beta Chemokine Exodus-2,Efficient Chemoattractant For Lymphocytes
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
Frequently bought together:

Description

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Human Secondary Lymphoid Tissue Chemokine (SLC) ELISA Kit

The Human Secondary Lymphoid Tissue Chemokine (SLC) ELISA Kit is designed for precise measurement of SLC levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, guaranteeing accurate and consistent results for various research applications.SLC, also known as CCL21, is a chemokine crucial for immune cell trafficking and lymphoid tissue organization. It plays a vital role in immune responses, inflammation, and autoimmune diseases.

The detection of SLC levels can provide valuable insights into immune system function and disease pathogenesis.This ELISA kit provides a reliable tool for studying the role of SLC in immune regulation and disease development, facilitating the discovery of potential therapeutic targets and strategies. With its high performance and user-friendly format, the Human Secondary Lymphoid Tissue Chemokine ELISA Kit is an essential asset for immunology and inflammation research.

Product Name:Human Secondary Lymphoid Tissue Chemokine (SLC) ELISA Kit
Product Code:HUDL02711
Size:96 Assays
Target:SLC
Synonyms:CCL21, 6Ckine, CKb9, ECL, SCYA21, TCA4, ECL, Chemokine(C-C-Motif)Ligand 21, Beta Chemokine Exodus-2, Efficient Chemoattractant For Lymphocytes
Detection Method:Sandwich
Range:31.25-2000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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