SIR2L5,Silent Mating Type Information Regulation 2 Homolog 5,NAD-dependent protein deacylase sirtuin-5, mitochondrial,Regulatory protein SIR2 homolog 5,SIR2-like protein 5
The Human Sirtuin-5 (SIRT5) ELISA Kit is specifically designed for the accurate detection of SIRT5 levels in human samples such as serum, plasma, and cell culture supernatants. With high sensitivity and specificity, this kit ensures reliable and reproducible results, making it perfect for various research applications.SIRT5 is a key enzyme belonging to the sirtuin family, known for its role in regulating various cellular processes such as metabolism, DNA damage repair, and stress response.
Dysregulation of SIRT5 has been linked to a variety of diseases including cancer, metabolic disorders, and neurodegenerative conditions, making it a valuable biomarker for understanding these diseases and potentially developing targeted therapies.With the Human Sirtuin-5 ELISA Kit, researchers can accurately measure SIRT5 levels in biological samples, providing valuable insights into its biological functions and potential therapeutic applications.
Product Name:
Human Sirtuin 5 (SIRT5) ELISA Kit
Product Code:
HUDL02691
Size:
96 Assays
Target:
SIRT5
Synonyms:
SIR2L5, Silent Mating Type Information Regulation 2 Homolog 5, NAD-dependent protein deacylase sirtuin-5, mitochondrial, Regulatory protein SIR2 homolog 5, SIR2-like protein 5
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
