The Human SIRT1 (Sirtuin 1) ELISA Kit is specifically designed for the precise measurement of Sirtuin 1 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.Sirtuin 1 is a key protein involved in regulating cellular processes such as metabolism, aging, and stress response. It plays a crucial role in various physiological and pathological conditions, including metabolic disorders, neurodegenerative diseases, and cancer.
The ability to accurately measure Sirtuin 1 levels can provide valuable insights into these conditions and aid in the development of potential treatment strategies.Overall, the Human SIRT1 ELISA Kit from Assay Genie is a reliable tool for researchers looking to study Sirtuin 1 and its impact on human health.
Product Name:
Human SIRT1 (Sirtuin 1) ELISA Kit
SKU:
HUES02560
Target:
Human SIRT1 (Sirtuin 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SIRT1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SIRT1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SIRT1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SIRT1. You can calculate the concentration of Human SIRT1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-102
88-103
99-110
Average (%)
94
94
104
1:4
Range (%)
89-103
84-96
86-102
Average (%)
94
90
93
1:8
Range (%)
86-102
83-96
83-96
Average (%)
93
89
90
1:16
Range (%)
92-104
83-96
88-100
Average (%)
98
89
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
90-104
97
EDTA plasma (n=5)
86-97
91
Cell culture media (n=5)
91-106
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.05
3.11
7.92
1.15
3.24
7.26
Standard deviation
0.06
0.18
0.32
0.06
0.13
0.33
C V (%)
5.71
5.79
4.04
5.22
4.01
4.55
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SIRT1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SIRT1 in samples. No significant cross-reactivity or interference between Human SIRT1 and analogues was observed.
