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Human Signal Transducer And Activator Of Transcription 1 (STAT1) ELISA Kit

SKU:
HUDL02783
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P42224
Sensitivity:
0.114ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
ISGF-3,STAT91,Transcription Factor ISGF-3 Components p91/p84
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Signal Transducer And Activator Of Transcription 1 (STAT1) ELISA Kit

The Human Signal Transducer and Activator of Transcription 1 (STAT1) ELISA Kit is a powerful tool for the accurate measurement of STAT1 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.STAT1 is a key protein involved in cellular signaling and immune response, playing a critical role in regulating gene expression and inflammatory processes.

Dysregulation of STAT1 has been linked to a range of diseases including cancer, autoimmune disorders, and infectious diseases, highlighting its importance as a potential biomarker for disease progression and therapeutic development.With its reliable performance and versatility, the Human STAT1 ELISA Kit is an essential resource for researchers studying the role of STAT1 in health and disease, offering valuable insights into the mechanisms underlying various pathological conditions.

Product Name:Human Signal Transducer And Activator Of Transcription 1 (STAT1) ELISA Kit
Product Code:HUDL02783
Size:96 Assays
Target:STAT1
Synonyms:ISGF-3, STAT91, Transcription Factor ISGF-3 Components p91/p84
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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