The Human SIGA (Secretory Immunoglobulin A) ELISA Kit is a cutting-edge tool designed for the accurate measurement of SIGA levels in human biological samples including serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring consistent and dependable results for various research applications.SIGA is an important component of the immune system, particularly in mucosal immunity, providing defense against pathogens in the mucous membranes of the respiratory, gastrointestinal, and genitourinary tracts.
Monitoring SIGA levels can offer valuable insights into the body's immune response and help researchers better understand conditions related to mucosal immunity, such as infections and autoimmune disorders.Overall, the Human SIGA ELISA Kit is a valuable resource for researchers studying immune responses, mucosal immunity, and infectious diseases, offering reliable data to advance scientific understanding and potentially lead to the development of novel therapeutic interventions.
Product Name:
Human sIgA (Secretory Immunoglobulin A) ELISA Kit
SKU:
HUES02333
Target:
Human sIgA (Secretory Immunoglobulin A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human sIgA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human sIgA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human sIgA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human sIgA. You can calculate the concentration of Human sIgA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
84-97
99-116
90-101
Average (%)
91
106
95
1:4
Range (%)
91-105
82-92
87-98
Average (%)
98
87
92
1:8
Range (%)
88-100
82-94
86-100
Average (%)
94
89
91
1:16
Range (%)
91-105
81-96
82-95
Average (%)
97
88
88
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-109
100
EDTA plasma (n=5)
93-108
100
Cell culture media (n=5)
84-98
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.04
3.12
7.12
1.12
2.98
6.44
Standard deviation
0.06
0.14
0.24
0.06
0.18
0.24
C V (%)
5.77
4.49
3.37
5.36
6.04
3.73
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human sIgA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human sIgA in samples. No significant cross-reactivity or interference between Human sIgA and analogues was observed.
