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Human Short Palate, Lung And Nasal Epithelium Carcinoma Associated Protein 2 (SPLUNC2) ELISA Kit

SKU:
HUDL02759
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q96DR5
Sensitivity:
0.059ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
BPIFA2,PSP,C20orf70,BPI fold-containing family A member 2,Parotid secretory protein
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
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Description

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Human Short Palate, Lung And Nasal Epithelium Carcinoma Associated Protein 2 (SPLUNC2) ELISA Kit

The Human SPLUNC2 (Short Palate, Lung, and Nasal Epithelium Carcinoma-Associated Protein 2) ELISA Kit is specifically designed for the accurate detection of SPLUNC2 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.SPLUNC2 is a protein that plays a key role in respiratory and immune function, with implications in lung diseases such as asthma, chronic obstructive pulmonary disease (COPD), and lung cancer.

By measuring SPLUNC2 levels, researchers can gain valuable insights into the pathogenesis of these conditions and potentially identify new therapeutic targets.Overall, the Human SPLUNC2 ELISA Kit is a valuable tool for studying the role of SPLUNC2 in respiratory diseases and advancing the development of targeted therapies for patients in need.

Product Name:Human Short Palate, Lung And Nasal Epithelium Carcinoma Associated Protein 2 (SPLUNC2) ELISA Kit
Product Code:HUDL02759
Size:96 Assays
Target:SPLUNC2
Synonyms:BPIFA2, PSP, C20orf70, BPI fold-containing family A member 2, Parotid secretory protein
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10191
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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