Human SFRP2 (Secreted Frizzled Related Protein 2) ELISA Kit
The Human SFRP2 (Secreted Frizzled-Related Protein 2) ELISA Kit is a comprehensive solution for the accurate measurement of SFRP2 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.SFRP2 is an important protein involved in various physiological processes, including the regulation of cell proliferation, migration, and tissue development.
Its dysregulation has been linked to conditions such as cancer, osteoarthritis, and fibrosis, making it a valuable biomarker for studying disease mechanisms and potential therapeutic interventions.With the Human SFRP2 ELISA Kit, researchers can easily quantify SFRP2 levels in biological samples, allowing for in-depth investigations into its roles in health and disease. Trust in the reliability and accuracy of this kit for your research needs.
Product Name:
Human SFRP2 (Secreted Frizzled Related Protein 2) ELISA Kit
SKU:
HUES02365
Target:
Human SFRP2 (Secreted Frizzled Related Protein 2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SFRP2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SFRP2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SFRP2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SFRP2. You can calculate the concentration of Human SFRP2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-100
99-111
91-106
Average (%)
92
104
97
1:4
Range (%)
91-101
82-98
88-101
Average (%)
96
89
93
1:8
Range (%)
93-108
81-92
84-94
Average (%)
99
86
89
1:16
Range (%)
90-106
84-94
89-104
Average (%)
98
89
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
92-110
100
EDTA plasma (n=5)
91-105
97
Cell culture media (n=5)
85-97
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
3.77
7.03
29.83
4.0
7.14
30.06
Standard deviation
0.19
0.31
1.27
0.27
0.4
1.05
C V (%)
5.04
4.41
4.26
6.75
5.6
3.49
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SFRP2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SFRP2 in samples. No significant cross-reactivity or interference between Human SFRP2 and analogues was observed.
