Human SF3B14 Recombinant Protein (RPPB4658)
- SKU:
- RPPB4658
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9Y3B4
Description
| Product Name: | Human SF3B14 Recombinant Protein |
| Product Code: | RPPB4658 |
| Size: | 10µg |
| Species: | Human |
| Target: | SF3B14 |
| Synonyms: | Pre-mRNA branch site protein p14, SF3b 14 kDa subunit, SF3B14, CGI-110, HSPC175, HT006, P14, SAP14, SF3B14a. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The SF3B14 solution (0.5 mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 0.2M NaCl, 5mM DTT, 1mM EDTA and�30% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.�Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).�Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAMQAAKRAN IRLPPEVNRI LYIRNLPYKI TAEEMYDIFG KYGPIRQIRV GNTPETRGTA YVVYEDIFDA KNACDHLSGF NVCNRYLVVL YYNANRAFQK MDTKKKEEQL KLLKEKYGIN TDPPK |
SF3B14 is a 125 amino acid nuclear protein which is a component of the splicing factor 3b complex. Splicing factor 3b is involved with s with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. SF3B14 which is required for the splicing of pre-mRNA enters the spliceosome and connect with the pre-mRNA branch site facilitating the interaction of snRNP with the branch sites of U2 and U12 of the 17S U2 and the 18S U11/U12 snRNP complex.
SF3B14 Human Recombinant fused with a�20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing�145 amino acids (1-125a.a.) and having a molecular mass of 16.7kDa. The SF3B14 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | SF3B6: subunit of the splicing factor SF3B. Necessary for the splicing of pre-mRNA. Directly contacts the pre-mRNA branch site adenosine for the first catalytic step of splicing. Enters the spliceosome and associates with the pre-mRNA branch site as part of the 17S U2 or, in the case of the minor spliceosome, as part of the 18S U11/U12 snRNP complex, and thus may facilitate the interaction of these snRNP with the branch sites of U2 and U12 respectively. |
| UniProt Protein Details: | Protein type:Spliceosome; RNA-binding; RNA splicing Chromosomal Location of Human Ortholog: 2p23 Cellular Component: nucleoplasm; snRNP U2; U12-dependent spliceosome Molecular Function:nucleotide binding Biological Process: blastocyst formation; gene expression; nuclear mRNA splicing, via spliceosome; RNA splicing |
| NCBI Summary: | This gene encodes a 14 kDa protein subunit of the splicing factor 3b complex. Splicing factor 3b associates with both the U2 and U11/U12 small nuclear ribonucleoprotein complexes (U2 snRNP) of spliceosomes. This 14 kDa protein interacts directly with subunit 1 of the splicing factor 3b complex. This 14 kDa protein also interacts directly with the adenosine that carries out the first transesterification step of splicing at the pre-mRNA branch site. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9Y3B4 |
| NCBI GenInfo Identifier: | 7706326 |
| NCBI Gene ID: | 51639 |
| NCBI Accession: | NP_057131.1 |
| UniProt Secondary Accession: | Q9Y3B4,Q53TM1, |
| UniProt Related Accession: | Q9Y3B4 |
| Molecular Weight: | 14,585 Da |
| NCBI Full Name: | splicing factor 3B subunit 6 |
| NCBI Synonym Full Names: | splicing factor 3b subunit 6 |
| NCBI Official Symbol: | SF3B6�� |
| NCBI Official Synonym Symbols: | P14; Ht006; SAP14; SAP14a; SF3B14; CGI-110; HSPC175; SF3B14a�� |
| NCBI Protein Information: | splicing factor 3B subunit 6 |
| UniProt Protein Name: | Splicing factor 3B subunit 6 |
| UniProt Synonym Protein Names: | Pre-mRNA branch site protein p14; SF3b 14 kDa subunit; SF3B14a; Spliceosome-associated protein, 14-kDa; Splicing factor 3b, subunit 6, 14kDa |
| Protein Family: | Splicing factor |
| UniProt Gene Name: | SF3B6�� |
| UniProt Entry Name: | SF3B6_HUMAN |
