Human Sex Determining Region Y Box Protein 9 (SOX9) ELISA Kit
The Human Sex-Determining Region Y-Box Protein 9 (SOX9) ELISA Kit is a powerful tool designed for the accurate and precise measurement of SOX9 levels in human samples such as serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring robust and reliable results for a variety of research applications.SOX9 is a key transcription factor that plays a critical role in the development and differentiation of multiple tissues, including the skeletal system and the male reproductive system. Dysregulation of SOX9 has been implicated in various diseases and conditions, making it a valuable biomarker for studying these disorders and exploring potential therapeutic interventions.
With the Human SOX9 ELISA Kit, researchers can confidently quantitate SOX9 levels in human samples, enabling deeper insights into the molecular mechanisms underlying diseases such as skeletal dysplasia, campomelic dysplasia, and prostate cancer. This kit offers a convenient and efficient way to study the role of SOX9 in health and disease, paving the way for advancements in personalized medicine and targeted therapies.
Product Name:
Human Sex Determining Region Y Box Protein 9 (SOX9) ELISA Kit
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
