Human Sex Determining Region Y Box Protein 18 (SOX18) ELISA Kit
The Human Sex Determining Region Y-Box Protein 18 (SOX18) ELISA Kit is a cutting-edge assay designed for the precise measurement of SOX18 levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for various research applications.SOX18 is a key transcription factor that plays a critical role in the development of blood vessels and lymphatic vessels. Dysregulation of SOX18 has been implicated in diseases such as cardiovascular disorders, lymphedema, and cancer.
Therefore, detecting and quantifying SOX18 levels can provide valuable insights into these conditions and aid in the development of potential therapeutic strategies.With its high performance and reliability, the Human SOX18 ELISA Kit from AssayGenie is an indispensable tool for researchers studying vascular biology, cancer, and other related fields. Trust in AssayGenie to deliver accurate and dependable results for your research needs.
Product Name:
Human Sex Determining Region Y Box Protein 18 (SOX18) ELISA Kit
Product Code:
HUDL02746
Size:
96 Assays
Target:
SOX18
Synonyms:
HLTS, SRY-Box 18
Detection Method:
Sandwich
Range:
0.156-10ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
83-100
91
EDTA plasma (n=5)
90-103
96
Heparin plasma (n=5)
89-102
95
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
