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Human SET Domain Containing Protein 7 (SETD7) ELISA Kit

SKU:
HUDL02654
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8WTS6
Sensitivity:
0.054ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
SET7,SET7/9,Set9,KMT7,Lysine N-methyltransferase 7,Histone H3-K4 methyltransferase SETD7,Histone-lysine N-methyltransferase SETD7
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
$899
Frequently bought together:

Description

system_update_altDatasheet

Human SET Domain Containing Protein 7 (SETD7) ELISA Kit

The Human SET Domain Containing Protein 7 (SETD7) ELISA Kit is a valuable tool for accurately quantifying levels of SETD7 in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for various research applications.SETD7 is a key protein that plays a role in regulating gene expression through histone methylation, impacting various biological processes such as cell growth, differentiation, and development.

Dysregulation of SETD7 has been implicated in diseases like cancer, diabetes, and cardiovascular disorders, underscoring the importance of studying its expression levels as a potential biomarker for disease diagnosis and therapeutic interventions.With its reliable performance and broad utility in scientific research, the Human SETD7 ELISA Kit is an indispensable tool for scientists and researchers investigating the role of SETD7 in health and disease.

Product Name:Human SET Domain Containing Protein 7 (SETD7) ELISA Kit
Product Code:HUDL02654
Size:96 Assays
Target:SETD7
Synonyms:SET7, SET7/9, Set9, KMT7, Lysine N-methyltransferase 7, Histone H3-K4 methyltransferase SETD7, Histone-lysine N-methyltransferase SETD7
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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