The Human SERT (Serotonin Transporter) CLIA Kit is specifically designed for the precise measurement of serotonin transporter levels in human samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.The serotonin transporter, also known as SERT, plays a vital role in the regulation of serotonin levels in the brain. It is involved in various physiological processes, including mood regulation, sleep, and appetite.
Dysregulation of SERT has been linked to disorders such as depression, anxiety, and obsessive-compulsive disorder, making it an important target for research and drug development.By accurately quantifying SERT levels, researchers can gain valuable insights into the role of serotonin in health and disease, paving the way for potential diagnostic and therapeutic advancements in mental health and neurological disorders. Trust the Human SERT CLIA Kit for reliable and reproducible results in your research endeavors.
Product Name:
Human SERT (Serotonin Transporter ) CLIA Kit
SKU:
HUES01154
Target:
Human SERT (Serotonin Transporter )
Size:
96T
Assay type:
Sandwich-CLIA
Assay time:
3.5h
Sensitivity:
4.69 pg/mL
Detection range:
7.81-500 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SERT. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SERT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SERT, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SERT. You can calculate the concentration of Human SERT in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
97-114
97-113
99-112
Average (%)
104
104
104
1:4
Range (%)
101-117
92-107
89-103
Average (%)
108
100
95
1:8
Range (%)
99-114
95-109
92-107
Average (%)
107
103
98
1:16
Range (%)
90-102
100-115
85-97
Average (%)
96
107
92
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
97-110
104
EDTA plasma (n=5)
99-114
105
Cell culture media (n=5)
85-100
91
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
24.96
62.66
215.31
25.34
67.64
219.3
Standard deviation
2.39
4.4
21.75
2.89
6.55
14.61
C V (%)
9.58
7.02
10.1
11.4
9.68
6.66
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SERT concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SERT in samples. No significant cross-reactivity or interference between Human SERT and analogues was observed.
