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Human Serine/Threonine Kinase 11 (STK11) ELISA Kit

SKU:
HUDL02796
Product Type:
ELISA Kit
Sensitivity:
0.071ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
LKB1,PJS,Polarization-Related Protein LKB1,Peutz-Jeghers Syndrome,Liver kinase B1,Renal carcinoma antigen NY-REN-19
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
$779
Frequently bought together:

Description

system_update_altDatasheet

Human Serine/Threonine Kinase 11 (STK11) ELISA Kit

The Human Serine/Threonine Kinase 11 (STK11) ELISA Kit is specifically designed for the precise measurement of STK11 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit provides accurate and reproducible results, making it an ideal tool for a wide range of research applications.STK11, also known as LKB1, is a critical protein involved in cell growth and metabolism regulation. Dysfunction of STK11 has been linked to various diseases, including cancer, metabolic disorders, and neurodegenerative diseases.

By detecting STK11 levels, researchers can gain insights into disease mechanisms and potentially identify new therapeutic targets.Overall, the Human STK11 ELISA Kit offers a reliable and efficient solution for studying the role of STK11 in health and disease, paving the way for advancements in biomedical research and drug development.

Product Name:Human Serine/Threonine Kinase 11 (STK11) ELISA Kit
Product Code:HUDL02796
Size:96 Assays
Target:STK11
Synonyms:LKB1, PJS, Polarization-Related Protein LKB1, Peutz-Jeghers Syndrome, Liver kinase B1, Renal carcinoma antigen NY-REN-19
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-10396
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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