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Human Serine Peptidase Inhibitor Kunitz Type 2 (SPINT2) ELISA Kit

SKU:
HUDL02758
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O43291
Sensitivity:
0.103ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
HAI2,Kop,PB,Placental Bikunin,Hepatocyte growth factor activator inhibitor type 2
Reactivity:
Human
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Serine Peptidase Inhibitor Kunitz Type 2 (SPINT2) ELISA Kit

The Human Serine Peptidase Inhibitor, Kunitz Type 2 (SPINT2) ELISA Kit is a valuable tool for the precise measurement of SPINT2 levels in human biological samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research purposes.SPINT2, a member of the Kunitz-type serine protease inhibitor family, plays a critical role in regulating protease activity and modulating various physiological processes.

Dysregulation of SPINT2 has been implicated in various diseases, including cancer, inflammatory disorders, and liver disease, highlighting its potential as a biomarker for disease progression and therapeutic targeting.With its user-friendly protocol and reliable performance, the Human SPINT2 ELISA Kit is a valuable asset for researchers exploring the function and clinical significance of SPINT2 in diverse biological systems.

Product Name:Human Serine Peptidase Inhibitor Kunitz Type 2 (SPINT2) ELISA Kit
Product Code:HUDL02758
Size:96 Assays
Target:SPINT2
Synonyms:HAI2, Kop, PB, Placental Bikunin, Hepatocyte growth factor activator inhibitor type 2
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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