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Human Serine Peptidase Inhibitor Kazal Type 5 (SPINK5) ELISA Kit

SKU:
HUDL02757
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q9NQ38
Sensitivity:
0.116ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
LEKTI,LETKI,NETS,NS,VAKTI,Lymphoepithelial Kazal-Type-Related Inhibitor,Lympho-epithelial Kazal-type-related inhibitor
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates And Other Biological Fluids
€649
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Description

system_update_altDatasheet

Human Serine Peptidase Inhibitor Kazal Type 5 (SPINK5) ELISA Kit

The Human Serine Peptidase Inhibitor, Kazal Type 5 (SPINK5) ELISA Kit is a reliable tool for detecting levels of SPINK5 in human samples including serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.SPINK5 is a critical protein involved in controlling protease activity and maintaining skin barrier function. Mutations in the SPINK5 gene have been linked to skin disorders such as Netherton syndrome, highlighting the importance of studying SPINK5 levels in understanding and treating these conditions.

By accurately measuring SPINK5 levels, researchers can gain valuable insights into skin barrier function and potential therapeutic targets for skin disorders. The Human SPINK5 ELISA kit provides a valuable tool for advancing research in this area and may lead to the development of novel treatments for skin conditions associated with SPINK5 dysfunction.

Product Name:Human Serine Peptidase Inhibitor Kazal Type 5 (SPINK5) ELISA Kit
Product Code:HUDL02757
Size:96 Assays
Target:SPINK5
Synonyms:LEKTI, LETKI, NETS, NS, VAKTI, Lymphoepithelial Kazal-Type-Related Inhibitor, Lympho-epithelial Kazal-type-related inhibitor
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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