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Human Serine Peptidase Inhibitor Kazal Type 1 (SPINK1) ELISA Kit

SKU:
HUDL02756
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P00995
Sensitivity:
0.62ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
PCTT,PSTI,TATI,Tumor-Associated Trypsin Inhibitor,Pancreatic secretory trypsin inhibitor,Tumor-associated trypsin inhibitor
Reactivity:
Human
Sample Type:
Serum, Plasma And Other Biological Fluids
$779
Frequently bought together:

Description

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Human Serine Peptidase Inhibitor Kazal Type 1 (SPINK1) ELISA Kit

The Human Serine Peptidase Inhibitor Kazal Type 1 (SPINK1) ELISA Kit is a powerful tool for the precise measurement of SPINK1 levels in human serum, plasma, and cell culture supernatants. This kit boasts exceptional sensitivity and specificity, guaranteeing accurate and consistent results for a variety of research applications.SPINK1 is a critical serine peptidase inhibitor that plays a key role in regulating various physiological processes, such as inflammation and tissue repair.

Dysregulation of SPINK1 has been linked to diseases like pancreatitis and cancer, underscoring its significance as a potential diagnostic and therapeutic target.With the Human SPINK1 ELISA Kit, researchers can gain valuable insights into the role of SPINK1 in disease pathogenesis and progression, paving the way for the development of novel treatment strategies. Trust in this advanced ELISA kit to deliver precise and reliable results for your research needs.

Product Name:Human Serine Peptidase Inhibitor Kazal Type 1 (SPINK1) ELISA Kit
Product Code:HUDL02756
Size:96 Assays
Target:SPINK1
Synonyms:PCTT, PSTI, TATI, Tumor-Associated Trypsin Inhibitor, Pancreatic secretory trypsin inhibitor, Tumor-associated trypsin inhibitor
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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