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Human Serine/Arginine Rich Splicing Factor 4 (SRSF4) ELISA Kit

SKU:
HUDL02774
Product Type:
ELISA Kit
Sensitivity:
0.058ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
SFRS4,SRP75,SRP001LB,Pre-mRNA-splicing factor SRP75,Splicing factor, arginine/serine-rich 4
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
$779
Frequently bought together:

Description

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Human Serine/Arginine Rich Splicing Factor 4 (SRSF4) ELISA Kit

The Human Serine/Arginine-Rich Splicing Factor 4 (SRSF4) ELISA Kit is a powerful tool for the precise measurement of SRSF4 levels in human biological samples such as serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for a variety of research applications.SRSF4 is a key protein involved in the process of RNA splicing, regulating gene expression and influencing cellular functions.

Dysregulation of SRSF4 has been linked to various diseases such as cancer, autoimmune disorders, and neurodegenerative diseases, highlighting its potential as a valuable biomarker for disease research and therapeutic development.With its advanced technology and reliable performance, the Human SRSF4 ELISA Kit is an essential tool for scientists and researchers studying the role of SRSF4 in disease pathogenesis and developing novel treatment strategies.

Product Name:Human Serine/Arginine Rich Splicing Factor 4 (SRSF4) ELISA Kit
Product Code:HUDL02774
Size:96 Assays
Target:SRSF4
Synonyms:SFRS4, SRP75, SRP001LB, Pre-mRNA-splicing factor SRP75, Splicing factor, arginine/serine-rich 4
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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