null

Human Sepiapterin Reductase (SPR) ELISA Kit

SKU:
HUDL02763
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P35270
Sensitivity:
0.108ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
SDR38C1,7,8-Dihydrobiopterin:NADP+ Oxidoreductase,Short Chain Dehydrogenase/Reductase Family 38C,Member 1
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Sepiapterin Reductase (SPR) ELISA Kit

The Human Sepiapterin Reductase (SPR) ELISA Kit is specially designed to accurately measure SPR levels in human samples such as serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures precise and consistent results, making it an ideal tool for a wide range of research applications.SPR is an enzyme that plays a crucial role in the biosynthesis of tetrahydrobiopterin (BH4), a cofactor essential for the function of several enzymes involved in neurotransmitter synthesis.

Dysregulation of SPR has been linked to various neurodevelopmental disorders and neurodegenerative diseases, making it a valuable biomarker for studying these conditions and potentially developing therapeutic interventions.Overall, the Human SPR ELISA Kit from Assay Genie is a reliable and efficient solution for researchers looking to investigate the role of SPR in health and disease.

Product Name:Human Sepiapterin Reductase (SPR) ELISA Kit
Product Code:HUDL02763
Size:96 Assays
Target:SPR
Synonyms:SDR38C1, 7,8-Dihydrobiopterin:NADP+ Oxidoreductase, Short Chain Dehydrogenase/Reductase Family 38C,Member 1
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-9990
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products