The Human sENG (Soluble Endoglin) ELISA Kit is specially designed for the precise measurement of soluble endoglin levels in human samples including serum, plasma, and cell culture supernatants. With exceptional sensitivity and specificity, this kit ensures accurate and consistent results, making it an invaluable tool for a variety of research applications.Soluble endoglin is a key regulator of angiogenesis and vascular function, playing a critical role in conditions such as preeclampsia, cardiovascular diseases, and diabetic complications.
By measuring soluble endoglin levels, researchers can gain valuable insights into these diseases and potentially identify new therapeutic targets for intervention. Don't miss out on the opportunity to advance your research with the Human sENG ELISA Kit from Assay Genie. Order yours today and uncover the secrets of soluble endoglin in human health and disease.
Product Name:
Human sENG (Soluble Endoglin) ELISA Kit
SKU:
HUES03402
Target:
Human sENG (Soluble Endoglin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.10 ng/mL
Detection range:
0.16-10 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human sENG. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human sENG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human sENG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human sENG. You can calculate the concentration of Human sENG in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
85-97
98-110
87-98
Average (%)
91
104
93
1:4
Range (%)
88-103
84-98
89-102
Average (%)
95
89
94
1:8
Range (%)
88-102
82-97
90-104
Average (%)
95
89
95
1:16
Range (%)
88-102
85-95
90-100
Average (%)
93
90
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
95-110
102
EDTA plasma (n=5)
89-102
95
Cell culture media (n=5)
91-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.47
1.21
3.98
0.46
1.19
4.06
Standard deviation
0.02
0.05
0.12
0.02
0.06
0.16
C V (%)
4.26
4.13
3.02
4.35
5.04
3.94
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human sENG concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human sENG in samples. No significant cross-reactivity or interference between Human sENG and analogues was observed.
