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Human Semaphorin 7A (SEMA7A) ELISA Kit

SKU:
HUDL02642
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
O75326
Sensitivity:
0.059ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
CD108,CDw108,H-SEMA-K1,H-Sema-L,JMH,SEMAK1,SEMAL,Semaphorin 7A,GPI Membrane Anchor,John Milton Hagen Blood Group,Semaphorin-K1,Semaphorin-L
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Semaphorin 7A (SEMA7A) ELISA Kit

The Human Semaphorin-7A (SEMA7A) ELISA Kit is designed for the precise measurement of SEMA7A levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring accurate and consistent results for various research applications.SEMA7A is a key protein that plays a crucial role in immune response modulation, cell migration, and axon guidance.

Dysregulation of SEMA7A has been linked to inflammatory diseases, cancer metastasis, and neuronal disorders, highlighting its importance as a potential biomarker for studying and developing treatments for these conditions.With its reliable performance and easy-to-use protocol, the Human SEMA7A ELISA Kit is a valuable tool for researchers investigating the role of SEMA7A in health and disease.

Product Name:Human Semaphorin 7A (SEMA7A) ELISA Kit
Product Code:HUDL02642
Size:96 Assays
Target:SEMA7A
Synonyms:CD108, CDw108, H-SEMA-K1, H-Sema-L, JMH, SEMAK1, SEMAL, Semaphorin 7A,GPI Membrane Anchor, John Milton Hagen Blood Group, Semaphorin-K1, Semaphorin-L
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)91-10095
Heparin plasma (n=5)91-10196
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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