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Human Semaphorin 3F (SEMA3F) ELISA Kit

SKU:
HUDL02637
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q13275
Sensitivity:
19.25pg/mL
Range:
46.875-3000pg/mL
ELISA Type:
Sandwich
Synonyms:
SEMA-IV,SEMA4,SEMAK,Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3F
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
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Description

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Human Semaphorin 3F (SEMA3F) ELISA Kit

The Human Semaphorin 3F (SEMA3F) ELISA Kit is a powerful tool for the precise measurement of SEMA3F levels in human samples, including serum, plasma, and cell culture supernatants. With its advanced sensitivity and specificity, this kit delivers accurate and consistent results, making it well-suited for various research purposes.SEMA3F is a key player in the regulation of neuronal development and axon guidance, as well as in immune responses and tumor progression. Its dysregulation has been implicated in various diseases, including cancer, autoimmune disorders, and neurological conditions.

Therefore, the detection of SEMA3F levels is crucial for understanding the mechanisms underlying these diseases and identifying potential therapeutic targets.In summary, the Human SEMA3F ELISA Kit provides researchers with a reliable tool for quantifying SEMA3F levels in human samples, enabling in-depth investigations into its role in health and disease.

Product Name:Human Semaphorin 3F (SEMA3F) ELISA Kit
Product Code:HUDL02637
Size:96 Assays
Target:SEMA3F
Synonyms:SEMA-IV, SEMA4, SEMAK, Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3F
Detection Method:Sandwich
Range:46.875-3,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)91-9894
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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