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Human Semaphorin 3A (SEMA3A) ELISA Kit

SKU:
HUDL02633
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q14563
Sensitivity:
0.57ng/mL
Range:
1.56-100ng/mL
ELISA Type:
Sandwich
Synonyms:
SEMAL,Hsema-I,Hsema-III,SEMA1,SEMAD,SEMAIII,SemD,Coll-1,Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3A
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€749
Frequently bought together:

Description

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Human Semaphorin 3A (SEMA3A) ELISA Kit

The Human Semaphorin 3A (SEMA3A) ELISA Kit is a reliable tool for the quantitative detection of SEMA3A levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring accurate and reproducible results for a variety of research applications.SEMA3A is a vital protein that plays a crucial role in axon guidance and neural development, making it a key player in neurobiology research. Dysregulation of SEMA3A has been implicated in various neurological disorders, making it a valuable biomarker for studying these conditions and developing potential therapeutic interventions.

Overall, the Human SEMA3A ELISA Kit is a valuable asset for researchers looking to investigate the role of SEMA3A in various physiological and pathological processes, providing a reliable tool for precise and accurate quantification of SEMA3A levels.

Product Name:Human Semaphorin 3A (SEMA3A) ELISA Kit
Product Code:HUDL02633
Size:96 Assays
Target:SEMA3A
Synonyms:SEMAL, Hsema-I, Hsema-III, SEMA1, SEMAD, SEMAIII, SemD, Coll-1, Sema Domain,Immunoglobulin Domain(Ig),Transmembrane Domain(TM)and Short Cytoplasmic Domain 3A
Detection Method:Sandwich
Range:1.56-100ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)91-9894
Heparin plasma (n=5)90-9994
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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