The Assay Genie Human SELP (P-Selectin) ELISA Kit is expertly designed to facilitate the precise quantitative measurement of P-Selectin levels in a variety of biological samples.
P-Selectin, also known as SELP, is a crucial adhesion molecule that plays a fundamental role in mediating leukocyte rolling and promoting interactions between leukocytes and endothelial cells during the inflammatory response. By accurately quantifying P-Selectin levels, researchers can gain valuable insights into the mechanisms underlying inflammation, immune responses, and various inflammatory disorders. Our SELP ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results for your investigations. Manufactured under stringent quality control standards, this kit delivers reliable performance and user-friendly protocols, making it an excellent choice for research applications. Trust Assay Genie's SELP ELISA Kit for dependable and precise quantification of P-Selectin, empowering your studies in immunology and beyond.
Product Name:
Human SELP (P-Selectin) ELISA Kit
SKU:
AEES00180
Target:
Human SELP (P-Selectin)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
18.75 pg/mL
Detection range:
31.25-2000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SELP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SELP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SELP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SELP. You can calculate the concentration of Human SELP in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-101
91-105
91-106
Average (%)
95
97
98
1:4
Range (%)
87-103
91-105
94-104
Average (%)
94
97
99
1:8
Range (%)
85-95
88-105
94-111
Average (%)
90
96
101
1:16
Range (%)
89-102
91-106
86-102
Average (%)
97
98
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
89-103
96
EDTA plasma (n=5)
91-106
98
Cell culture media (n=5)
88-103
95
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
103.46
280.93
920.89
97.11
291.68
870.13
Standard deviation
6.75
15.31
45.95
6.33
12.86
35.07
C V (%)
6.52
5.45
4.99
6.52
4.41
4.03
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SELP concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SELP in samples. No significant cross-reactivity or interference between Human SELP and analogues was observed.
