null

Human Selectin, Leukocyte (SELL) ELISA Kit

SKU:
HUDL02631
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P14151
Sensitivity:
0.068ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
CD62L,L-Selectin,SEL-L,LAM1,LECAM1,LNHR,LSEL,LYAM1,Leu-8,Lyam-1,PLNHR,TQ1,HLHRc,MEL14,Lymphocyte Adhesion Molecule 1,Lymph Node Homing Receptor
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates And Other Biological Fluids
€649
Frequently bought together:

Description

system_update_altDatasheet

Human Selectin, Leukocyte (SELL) ELISA Kit

The Human Selectin Leukocyte (SELL) ELISA Kit is a powerful tool for the precise measurement of SELL levels in human samples including serum, plasma, and cell culture supernatants. This kit provides exceptional sensitivity and specificity, ensuring accurate and dependable results for a variety of research experiments.Selectin Leukocyte (SELL) is a key protein involved in the adhesion of leukocytes to endothelial cells, playing a vital role in inflammation and immune response.

Dysregulation of SELL expression has been linked to various inflammatory diseases, making it a valuable marker for studying these conditions and exploring potential treatment options.With its user-friendly protocol and reliable performance, the Human Selectin Leukocyte (SELL) ELISA Kit is an essential tool for researchers investigating the role of SELL in health and disease. Order yours today from AssayGenie and take your research to the next level.

Product Name:Human Selectin, Leukocyte (SELL) ELISA Kit
Product Code:HUDL02631
Size:96 Assays
Target:SELL
Synonyms:CD62L, L-Selectin, SEL-L, LAM1, LECAM1, LNHR, LSEL, LYAM1, Leu-8, Lyam-1, PLNHR, TQ1, HLHRc, MEL14, Lymphocyte Adhesion Molecule 1, Lymph Node Homing Receptor
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

Related Products