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Human Selectin, Endothelium (SELE) ELISA Kit

SKU:
HUDL02629
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P16581
Sensitivity:
14pg/mL
Range:
39.062-2500pg/mL
ELISA Type:
Sandwich
Synonyms:
CD62E,CD62-E,E-Selectin,ELAM,ELAM1,ESEL,SEL-E,E-LAM,E-LAM1,E-SEL,LECAM2,Endothelial Leukocyte Adhesion Molecule 1,CD62 Antigen-Like Family Member E
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€599
Frequently bought together:

Description

system_update_altDatasheet

Human Selectin, Endothelium (SELE) ELISA Kit

The Human Selectin Endothelium (SELE) ELISA Kit is a powerful tool for the accurate detection of selectin endothelium levels in human samples such as serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring precise and reliable results for research purposes.Selectin endothelium, specifically SELE, is a cell adhesion molecule that plays a crucial role in inflammation and immune response by facilitating leukocyte rolling and adhesion to the endothelium.

Dysregulation of SELE expression has been implicated in various inflammatory conditions such as atherosclerosis, rheumatoid arthritis, and inflammatory bowel disease.By accurately measuring SELE levels, researchers can gain valuable insights into the pathogenesis of inflammatory diseases and potentially identify novel therapeutic targets. The Human Selectin Endothelium (SELE) ELISA Kit is an essential tool for studying these conditions and developing new treatment strategies.

Product Name:Human Selectin, Endothelium (SELE) ELISA Kit
Product Code:HUDL02629
Size:96 Assays
Target:SELE
Synonyms:CD62E, CD62-E, E-Selectin, ELAM, ELAM1, ESEL, SEL-E, E-LAM, E-LAM1, E-SEL, LECAM2, Endothelial Leukocyte Adhesion Molecule 1, CD62 Antigen-Like Family Member E
Detection Method:Sandwich
Range:39.0-2,500pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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