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Human Secretory Leukocyte Peptidase Inhibitor (SLPI) ELISA Kit

SKU:
HUDL02716
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P03973
Sensitivity:
25.8pg/mL
Range:
62.5-4000pg/mL
ELISA Type:
Sandwich
Synonyms:
ALK1,ALP,MPI,BLPI,HUSI,HUSI-I,WAP4,WFDC4,Antileukoproteinase,Seminal proteinase inhibitor,Mucus proteinase inhibitor,WAP four-disulfide core domain protein 4
Reactivity:
Human
Sample Type:
Serum, Plasma And Other Biological Fluids
€649
Frequently bought together:

Description

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Human Secretory Leukocyte Peptidase Inhibitor (SLPI) ELISA Kit

The Human Secretory Leukocyte Peptidase Inhibitor (SLPI) ELISA Kit is a valuable tool for researchers looking to accurately measure levels of SLPI in human samples such as serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and consistent results for a variety of research applications.SLPI is an important protein that plays a key role in immune responses and inflammation regulation. It is known to have antimicrobial, anti-inflammatory, and antiviral properties, making it a crucial biomarker in various diseases and conditions such as respiratory infections, inflammatory disorders, and cancer.

By using the Human SLPI ELISA Kit, researchers can gain valuable insights into the role of SLPI in disease progression and development, as well as explore potential therapeutic strategies targeting this protein. With its reliable performance and ease of use, this kit is an essential tool for advancing research in the field of immunology and inflammation.

Product Name:Human Secretory Leukocyte Peptidase Inhibitor (SLPI) ELISA Kit
Product Code:HUDL02716
Size:96 Assays
Target:SLPI
Synonyms:ALK1, ALP, MPI, BLPI, HUSI, HUSI-I, WAP4, WFDC4, Antileukoproteinase, Seminal proteinase inhibitor, Mucus proteinase inhibitor, WAP four-disulfide core domain protein 4
Detection Method:Sandwich
Range:62.5-4,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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