Human Secreted Frizzled Related Protein 5 (SFRP5) ELISA Kit
The Human Secreted Frizzled-Related Protein 5 (SFRP5) ELISA Kit is a reliable and accurate tool for the quantitative detection of SFRP5 levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.SFRP5 is a key player in the Wnt signaling pathway, acting as a secreted antagonist of frizzled receptors. It is involved in regulating cell proliferation, differentiation, and apoptosis, with implications in various diseases such as cancer, diabetes, and cardiovascular disorders.
The measurement of SFRP5 levels can provide valuable insights into disease progression and potential therapeutic interventions.With its user-friendly protocol and robust performance, the Human Secreted Frizzled-Related Protein 5 (SFRP5) ELISA Kit is an essential tool for researchers aiming to study the role of SFRP5 in health and disease. Trust in its accuracy and reliability to advance your research endeavors.
Product Name:
Human Secreted Frizzled Related Protein 5 (SFRP5) ELISA Kit
Product Code:
HUDL02661
Size:
96 Assays
Target:
SFRP5
Synonyms:
SARP3, Secreted Apoptosis Related Protein 3, Frizzled-related protein 1b
Detection Method:
Sandwich
Range:
1.56-100ng/mL
Shelf Life:
12 months
Note:
For research use only
Recovery:
Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
Matrix
Recovery range (%)
Average(%)
Serum (n=5)
80-102
91
EDTA plasma (n=5)
81-100
90
Heparin plasma (n=5)
80-89
84
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
1:16
Serum (n=5)
82-96%
83-98%
81-99%
93-101%
EDTA plasma (n=5)
88-101%
86-95%
90-102%
80-93%
Heparin plasma (n=5)
80-91%
82-90%
95-104%
79-95%
Intra-assay Precision:
Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:
Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Step
Protocol
1.
Prepare all reagents, samples and standards
2.
Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.
Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.
Aspirate and wash 3 times
5.
Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.
Aspirate and wash 5 times
7.
Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.
Add 50µL Stop Solution. Read at 450nm immediately.
