Human SCUBE1 (Signal Peptide, CUB and EGF-like Domain-containing Protein 1) ELISA Kit
The Human SCUBE1 (Signal Peptide, Cub, and EGF-like Domain Containing Protein 1) ELISA Kit is specifically designed for the precise measurement of SCUBE1 levels in human serum, plasma, and cell culture supernatants. This kit offers superior sensitivity and specificity, guaranteeing dependable and consistent results, making it well-suited for a diverse array of research purposes.SCUBE1 is a key protein that plays a critical role in various biological processes, including cell adhesion, proliferation, and differentiation.
It is associated with cardiovascular diseases, neurodegenerative disorders, and cancer, making it a valuable biomarker for the study of these conditions and the development of potential treatments.Overall, the Human SCUBE1 ELISA Kit from Assay Genie provides researchers with an essential tool for investigating the role of SCUBE1 in health and disease, offering accurate and reliable data for advancing scientific understanding and potential therapeutic interventions.
Product Name:
Human SCUBE1 (Signal Peptide, CUB and EGF-like Domain-containing Protein 1) ELISA Kit
SKU:
HUES03413
Target:
Human SCUBE1 (Signal Peptide, CUB and EGF-like Domain-containing Protein 1)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SCUBE1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SCUBE1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SCUBE1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SCUBE1. You can calculate the concentration of Human SCUBE1 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
93-107
85-101
96-112
Average (%)
98
92
102
1:4
Range (%)
89-101
86-98
86-100
Average (%)
95
92
93
1:8
Range (%)
87-100
83-94
85-98
Average (%)
94
89
90
1:16
Range (%)
89-101
80-94
84-97
Average (%)
96
86
91
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-95
90
EDTA plasma (n=5)
95-109
101
Cell culture media (n=5)
83-95
90
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
2
4.25
18.34
1.83
4.51
17.72
Standard deviation
0.11
0.25
0.97
0.11
0.21
0.7
C V (%)
5.5
5.88
5.29
6.01
4.66
3.95
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SCUBE1 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SCUBE1 in samples. No significant cross-reactivity or interference between Human SCUBE1 and analogues was observed.
