Human sCD14 (Soluble Cluster of Differentiation14) CLIA Kit
The Human SCD14 (Soluble Cluster of Differentiation 14) CLIA Kit is designed for the accurate detection of SCD14 levels in human serum, plasma, and cell culture supernatants. This kit features high sensitivity and specificity, ensuring reliable and reproducible results, making it ideal for a wide range of research applications.SCD14 is a protein associated with the immune system and is involved in the innate immune response to bacterial lipopolysaccharide (LPS). It plays a crucial role in inflammatory processes, sepsis, and various other immune-related disorders, making it an essential biomarker for studying these conditions and developing potential therapies.
With its easy-to-use format and high-quality components, the Human SCD14 CLIA Kit from AssayGenie is the perfect tool for researchers looking to accurately measure SCD14 levels in human samples. Get reliable results and advance your research with this innovative CLIA kit.
Product Name:
Human sCD14 (Soluble Cluster of Differentiation14) CLIA Kit
SKU:
HUES00033
Target:
Human sCD14 (Soluble Cluster of Differentiation14)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human sCD14. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human sCD14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human sCD14, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human sCD14. You can calculate the concentration of Human sCD14 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
99-116
97-112
95-113
Average (%)
106
103
103
1:4
Range (%)
88-103
90-106
103-121
Average (%)
94
98
110
1:8
Range (%)
97-112
94-109
98-113
Average (%)
104
100
104
1:16
Range (%)
102-117
96-114
96-110
Average (%)
108
104
104
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-105
97
EDTA plasma (n=5)
97-112
103
Cell culture media (n=5)
94-111
101
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
250.55
496.92
2301.51
252.74
451.57
2091.87
Standard deviation
28.56
58.49
211.28
31.95
34.18
164.84
C V (%)
11.4
11.77
9.18
12.64
7.57
7.88
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human sCD14 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human sCD14 in samples. No significant cross-reactivity or interference between Human sCD14 and analogues was observed.
