Human sCD14 (Soluble Cluster of Differentiation 14) ELISA Kit
The Human sCD14 (Soluble Cluster of Differentiation 14) ELISA Kit is meticulously designed for the precise and quantitative assessment of sCD14 levels in a variety of human biological samples. sCD14, a crucial protein known for its role in immune responses and inflammatory processes, serves as a key player in modulating immune reactions, specifically in sensing pathogens and activating immune cells. Accurate quantification of sCD14 levels is vital for unraveling its contributions to immune-related disorders, inflammatory conditions, and infectious diseases.
Our ELISA kit offers unparalleled sensitivity and specificity, ensuring the generation of reliable and reproducible data essential for exploring the diverse functions of sCD14. Manufactured under rigorous quality control standards, this kit delivers robust performance and user-friendly protocols, making it an optimal solution for cutting-edge research endeavors. Choose Assay Genie's sCD14 ELISA Kit for dependable and precise quantification of this significant biomarker in your research studies.
Product Name:
Human sCD14 (Soluble Cluster of Differentiation 14) ELISA Kit
SKU:
AEES00158
Target:
Human sCD14 (Soluble Cluster of Differentiation 14)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human sCD14. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human sCD14 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human sCD14, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human sCD14. You can calculate the concentration of Human sCD14 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
86-98
84-94
87-94
Average (%)
91
91
90
1:4
Range (%)
95-110
101-111
96-107
Average (%)
103
104
99
1:8
Range (%)
93-106
100-109
93-107
Average (%)
98
105
100
1:16
Range (%)
85-97
85-97
91-98
Average (%)
92
90
95
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
93-100
96
EDTA plasma (n=5)
86-100
94
Cell culture media (n=5)
85-99
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
3.3
8.64
39.05
3.34
8.89
37.01
Standard deviation
0.17
0.4
2.02
0.18
0.41
1.55
C V (%)
5.08
4.59
5.18
5.26
4.6
4.18
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human sCD14 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human sCD14 in samples. No significant cross-reactivity or interference between Human sCD14 and analogues was observed.
