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Human Scavenger Receptor Class A Member 3 (SCARA3) ELISA Kit

SKU:
HUDL02603
Product Type:
ELISA Kit
Sensitivity:
0.34ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
CSR1,CSR,MSLR1,APC7,MSRL1,Macrophage Scavenger Receptor-Like 1,Cellular stress response gene protein
Reactivity:
Human
Sample Type:
Tissue Homogenates And Other Biological Fluids
€649
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Description

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Human Scavenger Receptor Class A Member 3 (SCARA3) ELISA Kit

The Human SCARA3 (Scavenger Receptor Class A Member 3) ELISA Kit is a powerful tool for the accurate measurement of SCARA3 levels in human samples, including serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit delivers reliable and reproducible results, making it ideal for a variety of research applications.SCARA3 is a key receptor involved in various cellular processes, including lipid metabolism, inflammation, and immune response.

Its dysregulation has been linked to conditions such as atherosclerosis, Alzheimer's disease, and autoimmune disorders, highlighting its significance as a potential biomarker for studying and developing treatments for these diseases.Overall, the Human SCARA3 ELISA Kit provides researchers with a valuable tool for investigating the role of SCARA3 in health and disease, paving the way for new insights and therapeutic strategies.

Product Name:Human Scavenger Receptor Class A Member 3 (SCARA3) ELISA Kit
Product Code:HUDL02603
Size:96 Assays
Target:SCARA3
Synonyms:CSR1, CSR, MSLR1, APC7, MSRL1, Macrophage Scavenger Receptor-Like 1, Cellular stress response gene protein
Detection Method:Sandwich
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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