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Human SCARB1 / Scavenger receptor class B member 1 ELISA Kit

SKU:
HUFI01491
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
Q8WTV0
Sensitivity:
0.188ng/ml
Range:
0.313-20ng/ml
ELISA Type:
Sandwich
Synonyms:
SCARB1, CD36L1, CLA1, HDLQTL6, SR-B1, CD36L1, CLA1 CD36 antigen-like 1, CLA-1, Collagen type I receptor, thrombospondin receptor-like 1, scavenger receptor class B type III, scavenger receptor class B, member 1, SRB1 scavenger receptor class B member
Reactivity:
Human
Research Area:
Immunology
$719
Frequently bought together:

Description

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Human SCARB1/Scavenger receptor class B member 1 ELISA Kit

The Human SCARB1 (Scavenger Receptor Class B Member 1) ELISA Kit is specifically designed for the accurate measurement of SCARB1 levels in human serum, plasma, and cell culture supernatants. This kit offers exceptional sensitivity and specificity, ensuring precise and consistent results for a variety of research studies.SCARB1 is a vital protein that functions as a receptor for high-density lipoprotein (HDL) and plays a crucial role in cholesterol metabolism and lipid transport. Studies have shown that SCARB1 is implicated in various diseases, including cardiovascular disorders, metabolic syndromes, and atherosclerosis, making it a valuable target for research and potential therapeutic interventions.

With its advanced technology and reliable performance, the Human SCARB1 ELISA Kit is an indispensable tool for investigating the role of SCARB1 in disease pathology and exploring novel treatment strategies. Whether studying cardiovascular health, lipid metabolism, or related conditions, this kit provides accurate and reproducible results to advance your research efforts.

Product Name:Human SCARB1 / Scavenger receptor class B member 1 ELISA Kit
Product Code:HUFI01491
Size:96 Assays
Alias:SCARB1, CD36L1, CLA1, HDLQTL6, SR-B1, CD36L1, CLA1 CD36 antigen-like 1, CLA-1, Collagen type I receptor, thrombospondin receptor-like 1, scavenger receptor class B type III, scavenger receptor class B, member 1, SRB1 scavenger receptor class B member 1, SRBI
Detection method:Sandwich ELISA, Double Antibody
Application:This immunoassay kit allows for the in vitro quantitative determination of Human SCARB1 concentrations in serum plasma and other biological fluids.
Sensitivity:0.188ng/ml
Range:0.313-20ng/ml
Storage:4°C for 6 months
Note:For Research Use Only
Recovery:Matrices listed below were spiked with certain level of Human SCARB1 and the recovery rates were calculated by comparing the measured value to the expected amount of Human SCARB1 in samples.
MatrixRecovery range(%)Average(%)
serum(n=5)92-10397
EDTA plasma(n=5)89-10296
UFH plasma(n=5)90-10496
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human SCARB1 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample1:21:41:8
serum(n=5)85-105%86-97%87-105%
EDTA plasma(n=5)85-95%87-101%84-95%
UFH plasma(n=5)80-91%86-98%80-100%
CV(%):Intra-Assay: CV<8%
Inter-Assay: CV<10%
ComponentQuantityStorage
ELISA Microplate (Dismountable)8×12 strips4°C for 6 months
Lyophilized Standard24°C/-20°C
Sample/Standard Dilution Buffer20ml4°C
Biotin-labeled Antibody(Concentrated)120ul4°C (Protect from light)
Antibody Dilution Buffer10ml4°C
HRP-Streptavidin Conjugate(SABC)120ul4°C (Protect from light)
SABC Dilution Buffer10ml4°C
TMB Substrate10ml4°C (Protect from light)
Stop Solution10ml4°C
Wash Buffer(25X)30ml4°C
Plate Sealer5-

Other materials and equipment required:

  • Microplate reader with 450 nm wavelength filter
  • Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
  • Incubator
  • Deionized or distilled water
  • Absorbent paper
  • Buffer resevoir
UniprotQ8WTV0
UniProt Protein Function:SCARB1: Receptor for different ligands such as phospholipids, cholesterol ester, lipoproteins, phosphatidylserine and apoptotic cells. Probable receptor for HDL, located in particular region of the plasma membrane, called caveolae. Facilitates the flux of free and esterified cholesterol between the cell surface and extracellular donors and acceptors, such as HDL and to a lesser extent, apoB-containing lipoproteins and modified lipoproteins. Probably involved in the phagocytosis of apoptotic cells, via its phosphatidylserine binding activity. Receptor for hepatitis C virus glycoprotein E2. Binding between SCARB1 and E2 was found to be independent of the genotype of the viral isolate. Plays an important role in the uptake of HDL cholesteryl ester. Belongs to the CD36 family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:Protein type:Membrane protein, integral; Receptor, misc.; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12q24.31

Cellular Component: cell surface; lysosomal membrane; microvillus membrane; integral to plasma membrane; plasma membrane; caveola

Molecular Function:apolipoprotein A-I binding; protein binding; protein homodimerization activity; lipopolysaccharide binding; transporter activity; phosphatidylserine binding; low-density lipoprotein binding; lipopolysaccharide receptor activity; apolipoprotein binding; high-density lipoprotein binding; phosphatidylinositol binding

Biological Process: receptor-mediated endocytosis; wound healing; blood vessel endothelial cell migration; viral reproduction; positive regulation of nitric-oxide synthase activity; lipoprotein metabolic process; cholesterol efflux; androgen biosynthetic process; regulation of phagocytosis; recognition of apoptotic cell; lipopolysaccharide transport; cholesterol homeostasis; reverse cholesterol transport; detection of lipopolysaccharide; endothelial cell proliferation; lipopolysaccharide-mediated signaling pathway; adhesion to host; phospholipid transport; cholesterol catabolic process; cell adhesion

Disease: High Density Lipoprotein Cholesterol Level Quantitative Trait Locus 6

NCBI Summary:The protein encoded by this gene is a plasma membrane receptor for high density lipoprotein cholesterol (HDL). The encoded protein mediates cholesterol transfer to and from HDL. In addition, this protein is a receptor for hepatitis C virus glycoprotein E2. Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2019]
UniProt Code:Q8WTV0
NCBI GenInfo Identifier:37999904
NCBI Gene ID:949
NCBI Accession:Q8WTV0.1
UniProt Related Accession:Q8WTV0
Molecular Weight:~ 80kDa
NCBI Full Name:Scavenger receptor class B member 1
NCBI Synonym Full Names:scavenger receptor class B member 1
NCBI Official Symbol:SCARB1  
NCBI Official Synonym Symbols:CLA1; SRB1; CLA-1; SR-BI; CD36L1; HDLQTL6  
NCBI Protein Information:scavenger receptor class B member 1
UniProt Protein Name:Scavenger receptor class B member 1
UniProt Synonym Protein Names:CD36 and LIMPII analogous 1; CLA-1; CD36 antigen-like 1; Collagen type I receptor, thrombospondin receptor-like 1; SR-BI; CD_antigen: CD36
Protein Family:Scavenger receptor
UniProt Gene Name:SCARB1  
UniProt Entry Name:SCRB1_HUMAN

*Note: Protocols are specific to each batch/lot. For the correct instructions please follow the protocol included in your kit.

Before adding to wells, equilibrate the SABC working solution and TMB substrate for at least 30 min at 37°C. When diluting samples and reagents, they must be mixed completely and evenly. It is recommended to plot a standard curve for each test.

StepProtocol
1.Set standard, test sample and control (zero) wells on the pre-coated plate respectively, and then, record their positions. It is recommended to measure each standard and sample in duplicate. Wash plate 2 times before adding standard, sample and control (zero) wells!
2.Aliquot 0.1ml standard solutions into the standard wells.
3.Add 0.1 ml of Sample / Standard dilution buffer into the control (zero) well.
4.Add 0.1 ml of properly diluted sample ( Human serum, plasma, tissue homogenates and other biological fluids.) into test sample wells.
5.Seal the plate with a cover and incubate at 37 °C for 90 min.
6.Remove the cover and discard the plate content, clap the plate on the absorbent filter papers or other absorbent material. Do NOT let the wells completely dry at any time. Wash plate X2.
7.Add 0.1 ml of Biotin- detection antibody working solution into the above wells (standard, test sample & zero wells). Add the solution at the bottom of each well without touching the side wall.
8.Seal the plate with a cover and incubate at 37°C for 60 min.
9.Remove the cover, and wash plate 3 times with Wash buffer. Let wash buffer rest in wells for 1 min between each wash.
10.Add 0.1 ml of SABC working solution into each well, cover the plate and incubate at 37°C for 30 min.
11.Remove the cover and wash plate 5 times with Wash buffer, and each time let the wash buffer stay in the wells for 1-2 min.
12.Add 90 µl of TMB substrate into each well, cover the plate and incubate at 37°C in dark within 10-20 min. (Note: This incubation time is for reference use only, the optimal time should be determined by end user.) And the shades of blue can be seen in the first 3-4 wells (with most concentrated standard solutions), the other wells show no obvious color.
13.Add 50 µl of Stop solution into each well and mix thoroughly. The color changes into yellow immediately.
14. Read the O.D. absorbance at 450 nm in a microplate reader immediately after adding the stop solution.

When carrying out an ELISA assay it is important to prepare your samples in order to achieve the best possible results. Below we have a list of procedures for the preparation of samples for different sample types.

Sample TypeProtocol
SerumIf using serum separator tubes, allow samples to clot for 30 minutes at room temperature. Centrifuge for 10 minutes at 1,000x g. Collect the serum fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

If serum separator tubes are not being used, allow samples to clot overnight at 2-8°C. Centrifuge for 10 minutes at 1,000x g. Remove serum and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles.

PlasmaCollect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples at 4°C for 15 mins at 1000 × g within 30 mins of collection. Collect the plasma fraction and assay promptly or aliquot and store the samples at -80°C. Avoid multiple freeze-thaw cycles. Note: Over haemolysed samples are not suitable for use with this kit.
Urine & Cerebrospinal FluidCollect the urine (mid-stream) in a sterile container, centrifuge for 20 mins at 2000-3000 rpm. Remove supernatant and assay immediately. If any precipitation is detected, repeat the centrifugation step. A similar protocol can be used for cerebrospinal fluid.
Cell culture supernatantCollect the cell culture media by pipette, followed by centrifugation at 4°C for 20 mins at 1500 rpm. Collect the clear supernatant and assay immediately.
Cell lysatesSolubilize cells in lysis buffer and allow to sit on ice for 30 minutes. Centrifuge tubes at 14,000 x g for 5 minutes to remove insoluble material. Aliquot the supernatant into a new tube and discard the remaining whole cell extract. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Tissue homogenatesThe preparation of tissue homogenates will vary depending upon tissue type. Rinse tissue with 1X PBS to remove excess blood & homogenize in 20ml of 1X PBS (including protease inhibitors) and store overnight at ≤ -20°C. Two freeze-thaw cycles are required to break the cell membranes. To further disrupt the cell membranes you can sonicate the samples. Centrifuge homogenates for 5 mins at 5000xg. Remove the supernatant and assay immediately or aliquot and store at -20°C or -80°C.
Tissue lysatesRinse tissue with PBS, cut into 1-2 mm pieces, and homogenize with a tissue homogenizer in PBS. Add an equal volume of RIPA buffer containing protease inhibitors and lyse tissues at room temperature for 30 minutes with gentle agitation. Centrifuge to remove debris. Quantify total protein concentration using a total protein assay. Assay immediately or aliquot and store at ≤ -20 °C.
Breast MilkCollect milk samples and centrifuge at 10,000 x g for 60 min at 4°C. Aliquot the supernatant and assay. For long term use, store samples at -80°C. Minimize freeze/thaw cycles.

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