Human SAP18 Recombinant Protein (RPPB4583)
- SKU:
- RPPB4583
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- O00422
Description
| Product Name: | Human SAP18 Recombinant Protein |
| Product Code: | RPPB4583 |
| Size: | 20µg |
| Species: | Human |
| Target: | SAP18 |
| Synonyms: | Histone deacetylase complex subunit SAP18, Sin3-associated polypeptide 18 kDa, Sin3-associated polypeptide p18, Cell growth-inhibiting protein 38, 2HOR0202, SAP18, GIG38. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The SAP18 solution contains 20mM Tris-HCl buffer (pH8.0), 0.1M NaCl, 30% glycerol, 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAVESRVTQE EIKKEPEKPI DREKTCPLLL RVFTTNNGRH HRMDEFSRGN VPSSELQIYT WMDATLKELT SLVKEVYPEA RKKGTHFNFA IVFTDVKRPG YRVKEIGSTM SGRKGTDDSM TLQSQKFQIG DYLDIAITPP NRAPPPSGRM RPY |
SAP18 is a component of the histone deacetylase complex which has a significant role in the regulation of eukaryotic gene expression. SAP18 directly interacts with SIN3 and enhances SIN3-mediated transcriptional repression once bound to the promoter. SAP18 plays an important part in gene-specific recruitment of the HDAC complex by a number of transcription factors including Gli, GAGA, and Bicoid. Histone acetylation is significant in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation is catalyzed by multisubunit complexes. SAP18 is a component of the histone deacetylase complex, which includes SIN3, SAP30, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. SAP18 is a component of a splicing-dependent multiprotein EJC (exon junction complex) placed at splice junction on mRNAs.
SAP18 Human Recombinant fused with 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 173 amino acids (20- 172 a.a.) and having a molecular mass of 19.7 kDa.The SAP18 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | SAP18: Component of the SIN3-repressing complex. Enhances the ability of SIN3-HDAC1-mediated transcriptional repression. When tethered to the promoter, it can direct the formation of a repressive complex to core histone proteins. Component of a splicing-dependent multiprotein exon junction complex (EJC) deposited at splice junction on mRNAs. The EJC is a dynamic structure consisting of a few core proteins and several more peripheral nuclear and cytoplasmic associated factors that join the complex only transiently either during EJC assembly or during subsequent mRNA metabolism. Belongs to the SAP18 family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 13q12.11 Cellular Component: nucleoplasm; histone deacetylase complex; cytoplasm; nuclear speck Molecular Function:protein binding; transcription corepressor activity Biological Process: regulation of transcription from RNA polymerase II promoter; establishment and/or maintenance of chromatin architecture; negative regulation of nuclear mRNA splicing, via spliceosome; transcription, DNA-dependent; negative regulation of gene expression, epigenetic; positive regulation of apoptosis; RNA splicing; gene expression; mRNA processing; regulation of gene expression, epigenetic; regulation of alternative nuclear mRNA splicing, via spliceosome |
| NCBI Summary: | Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP30, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This protein directly interacts with SIN3 and enhances SIN3-mediated transcriptional repression when tethered to the promoter. A pseudogene has been identified on chromosome 2. [provided by RefSeq, Dec 2008] |
| UniProt Code: | O00422 |
| NCBI GenInfo Identifier: | 6831678 |
| NCBI Gene ID: | 10284 |
| NCBI Accession: | O00422.1 |
| UniProt Related Accession: | O00422 |
| Molecular Weight: | |
| NCBI Full Name: | Histone deacetylase complex subunit SAP18 |
| NCBI Synonym Full Names: | Sin3A associated protein 18 |
| NCBI Official Symbol: | SAP18�� |
| NCBI Official Synonym Symbols: | SAP18P; 2HOR0202�� |
| NCBI Protein Information: | histone deacetylase complex subunit SAP18 |
| UniProt Protein Name: | Histone deacetylase complex subunit SAP18 |
| UniProt Synonym Protein Names: | 18 kDa Sin3-associated polypeptide; 2HOR0202; Cell growth-inhibiting gene 38 protein; Sin3-associated polypeptide p18 |
| Protein Family: | Zinc finger A20 domain-containing stress-associated protein |
| UniProt Gene Name: | SAP18�� |
| UniProt Entry Name: | SAP18_HUMAN |
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