The Human SAA4 (Serum Amyloid A4) ELISA Kit is a powerful tool for the quantitative measurement of SAA4 levels in human serum samples. This kit offers exceptional sensitivity and specificity, ensuring accurate and reliable results for various research applications.SAA4 is a member of the Serum Amyloid A family, known for its involvement in inflammation and immune response. Elevated levels of SAA4 have been linked to a range of diseases, including cardiovascular conditions, rheumatoid arthritis, and inflammatory disorders.
Therefore, this ELISA kit serves as a valuable tool for studying the role of SAA4 in disease progression and developing potential therapeutic interventions.Overall, the Human SAA4 ELISA Kit from Assay Genie provides researchers with a robust assay to investigate the role of SAA4 in human health and disease, offering a valuable resource for advancing scientific understanding and clinical applications.
Product Name:
Human SAA4 (Serum Amyloid A4) ELISA Kit
SKU:
HUES03609
Target:
Human SAA4 (Serum Amyloid A4)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
46.88 pg/mL
Detection range:
78.13-5000 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SAA4. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SAA4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SAA4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SAA4. You can calculate the concentration of Human SAA4 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
88-104
96-111
88-102
Average (%)
96
102
93
1:4
Range (%)
92-104
84-96
81-96
Average (%)
99
90
88
1:8
Range (%)
87-102
81-97
81-92
Average (%)
94
88
87
1:16
Range (%)
90-103
79-91
84-95
Average (%)
96
86
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
91-102
96
EDTA plasma (n=5)
93-107
98
Cell culture media (n=5)
89-101
96
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
246.09
705.23
1803.13
228.39
762.72
1806.67
Standard deviation
12.58
33.22
77.35
12.15
41.19
72.99
C V (%)
5.11
4.71
4.29
5.32
5.4
4.04
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SAA4 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SAA4 in samples. No significant cross-reactivity or interference between Human SAA4 and analogues was observed.
