The Human Serum Amyloid A2 (SAA2) ELISA Kit is a powerful tool designed for the precise quantification of SAA2 levels in a variety of human biological samples. SAA2 is a key acute phase protein primarily produced by the liver in response to inflammatory stimuli and is involved in immune response modulation and lipid metabolism regulation. Elevated SAA2 levels are associated with various inflammatory conditions and pathologies, making its accurate measurement essential for understanding disease pathogenesis.
Assay Genie's SAA2 ELISA Kit offers exceptional sensitivity and specificity, ensuring accurate and reproducible results vital for investigating SAA2's role in health and disease. With rigorous quality control measures in place, this kit ensures reliable performance and ease of use, making it an optimal choice for research applications. Trust Assay Genie's SAA2 ELISA Kit to deliver dependable quantification of this important biomarker in your scientific investigations.
Product Name:
Human SAA2 (Serum Amyloid A2) ELISA Kit
SKU:
AEES00144
Target:
Human SAA2 (Serum Amyloid A2)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.19 ng/mL
Detection range:
0.31-20 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SAA2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SAA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SAA2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SAA2. You can calculate the concentration of Human SAA2 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
87-97
89-100
93-107
Average (%)
94
95
100
1:4
Range (%)
88-99
94-103
94-109
Average (%)
91
100
102
1:8
Range (%)
88-96
88-98
90-106
Average (%)
93
91
98
1:16
Range (%)
96-108
85-101
87-98
Average (%)
100
93
94
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
96-105
101
EDTA plasma (n=5)
95-107
102
Cell culture media (n=5)
85-95
92
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
0.85
2.19
8.13
0.88
2.18
9.48
Standard deviation
0.04
0.11
0.42
0.05
0.11
0.41
C V (%)
4.71
5.02
5.17
5.68
5.05
4.32
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SAA2 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SAA2 in samples. No significant cross-reactivity or interference between Human SAA2 and analogues was observed.
