The Human SAA2 (Serum Amyloid A2) CLIA Kit is a powerful tool for the quantitative measurement of SAA2 levels in human serum samples. This kit offers precise and accurate results, allowing researchers to investigate the role of SAA2 in various inflammatory and immune-related conditions.SAA2 is an acute phase protein that is produced in response to inflammation and infection. Elevated levels of SAA2 have been associated with a wide range of diseases, including rheumatoid arthritis, atherosclerosis, and sepsis.
By measuring SAA2 levels, researchers can gain valuable insights into the pathogenesis of these conditions and potentially identify new therapeutic targets.Overall, the Human SAA2 CLIA Kit from Assay Genie provides researchers with a reliable and efficient solution for studying the role of SAA2 in human health and disease. Its high sensitivity and specificity ensure accurate results, making it an essential tool for any laboratory conducting research in the field of inflammation and immunity.
Product Name:
Human SAA2 (Serum Amyloid A2) CLIA Kit
SKU:
HUES01228
Target:
Human SAA2 (Serum Amyloid A2)
Size:
96T
Assay type:
Sandwich
Assay time:
4.5h
Sensitivity:
7.50 pg/mL
Detection range:
12.50-800 pg/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
CLIA Plate
8 wells x 3 strips
8 wells x 12 strips
-20°C, 6 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 6 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 6 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent A
1 vial, 5 mL
1 vial, 5 mL
4°C (shading light)
Substrate Reagent B
1 vial, 5 mL
1 vial, 5 mL
Desiccant
1
1
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Human SAA2. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SAA2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SAA2, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Human SAA2. You can calculate the concentration of Human SAA2 in the samples by comparing the RLU value of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
90-104
96-109
92-108
Average (%)
96
101
99
1:4
Range (%)
86-100
88-100
101-114
Average (%)
92
94
107
1:8
Range (%)
87-100
98-111
94-108
Average (%)
94
103
101
1:16
Range (%)
86-98
100-113
102-118
Average (%)
91
107
108
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
100-115
105
EDTA plasma (n=5)
97-111
103
Cell culture media (n=5)
97-110
105
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20.0
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
44.34
83.51
334.56
48.29
84.79
328.13
Standard deviation
3.6
9.51
31.82
5.53
6.56
23.92
C V (%)
8.12
11.39
9.51
11.45
7.74
7.29
Sample type &Sample volume:
Serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 15%.
Application:
This CLIA kit applies to the in vitro quantitative determination of Human SAA2 concentrations in Serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SAA2 in samples. No significant cross-reactivity or interference between Human SAA2 and analogues was observed.
