The Human SAA (Serum Amyloid A) ELISA Kit is specifically designed for the precise measurement of SAA levels in human serum samples. This kit boasts exceptional sensitivity and specificity, guaranteeing dependable and consistent results that are suitable for various research purposes.SAA is a vital acute-phase protein that is implicated in inflammatory responses and is indicative of systemic inflammation. Elevated levels of SAA have been linked to various conditions such as rheumatoid arthritis, atherosclerosis, and cardiovascular diseases, underscoring its significance as a biomarker for studying these diseases and exploring therapeutic interventions.
With the Human SAA ELISA Kit, researchers can accurately quantitate SAA levels in human samples, facilitating the investigation of inflammatory processes and the development of potential diagnostic and therapeutic strategies.
Product Name:
Human SAA (Serum Amyloid A) ELISA Kit
SKU:
HUES03080
Target:
Human SAA (Serum Amyloid A)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.75 ng/mL
Detection range:
1.25-80 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SAA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SAA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SAA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SAA. You can calculate the concentration of Human SAA in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
89-102
93-108
91-105
Average (%)
96
100
98
1:4
Range (%)
87-99
81-94
89-100
Average (%)
93
87
94
1:8
Range (%)
90-102
87-97
85-97
Average (%)
96
92
90
1:16
Range (%)
90-103
83-96
84-97
Average (%)
95
90
90
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
84-95
90
EDTA plasma (n=5)
89-100
94
Cell culture media (n=5)
92-103
97
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
3.7
8.07
35.34
4.02
8.84
36.42
Standard deviation
0.23
0.46
1.55
0.21
0.43
1.65
C V (%)
6.22
5.7
4.39
5.22
4.86
4.53
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human SAA concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human SAA in samples. No significant cross-reactivity or interference between Human SAA and analogues was observed.
