Human S100A8 (S100 Calcium Binding Protein A8) ELISA Kit
The Human S100A8 (S100 Calcium Binding Protein A8) ELISA Kit is a reliable and accurate tool for the detection of S100A8 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is known for its high sensitivity and specificity, delivering consistent and precise results for a variety of research applications.S100A8, also known as S100 Calcium Binding Protein A8, is a vital protein involved in inflammatory processes and immune responses. It plays a crucial role in various diseases and conditions such as inflammatory disorders, autoimmune diseases, and cancer.
The measurement of S100A8 levels can provide valuable insights into the pathogenesis of these conditions and aid in the development of targeted therapies.Overall, the Human S100A8 ELISA Kit is an essential tool for researchers studying inflammation, immune responses, and related diseases, offering reliable and accurate quantification of S100A8 levels in human samples.
Product Name:
Human S100A8 (S100 Calcium Binding Protein A8) ELISA Kit
SKU:
HUES02345
Target:
Human S100A8 (S100 Calcium Binding Protein A8)
Size:
96T
Assay type:
Sandwich-ELISA
Assay time:
3.5h
Sensitivity:
0.38 ng/mL
Detection range:
0.63-40 ng/mL
Kit component:
Component
Quantity (24 Assays)
Quantity (96 Assays)
Storage
Micro ELISA Plate (Dismountable)
8 wells x 3 strips
8 wells x 12 strips
-20°C, 12 months
Reference Standard
1 vial
2 vials
Concentrated Biotinylated Detection Ab (100×)
1 vial, 60 µL
1 vial, 120 µL
Concentrated HRP Conjugate (100×)
1 vial, 60 µL
1 vial, 120 µL
-20°C (shading light), 12 months
Reference Standard & Sample Diluent
1 vial, 20 mL
1 vial, 20 mL
4°C, 12 months
Biotinylated Detection Ab Diluent
1 vial, 14 mL
1 vial, 14 mL
HRP Conjugate Diluent
1 vial, 14 mL
1 vial, 14 mL
Concentrated Wash Buffer (25×)
1 vial, 30 mL
1 vial, 30 mL
Substrate Reagent
1 vial, 10 mL
1 vial, 10 mL
4°C (shading light)
Stop Solution
1 vial, 10 mL
1 vial, 10 mL
4°C
Plate Sealer
5 pieces
5 pieces
Product Description
1 copy
1 copy
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human S100A8. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human S100A8 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human S100A8, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human S100A8. You can calculate the concentration of Human S100A8 in the samples by comparing the OD of the samples to the standard curve.
Linearity:
Serum (n=5)
EDTA plasma (n=5)
Cell culture media (n=5)
1:2
Range (%)
91-104
93-108
90-102
Average (%)
99
100
96
1:4
Range (%)
94-108
80-91
84-96
Average (%)
100
87
89
1:8
Range (%)
91-108
83-96
81-94
Average (%)
99
89
87
1:16
Range (%)
91-106
81-92
88-101
Average (%)
98
88
93
Recovery:
Sample Type
Range (%)
Average Recovery (%)
Serum (n=5)
94-106
101
EDTA plasma (n=5)
90-102
95
Cell culture media (n=5)
93-107
99
Precision:
Intra-assay Precision
Inter-assay Precision
Sample
1
2
3
1
2
3
n
20
20.0
20.0
20.0
20.0
20.0
Mean (ng/mL)
1.93
6.22
16.7
2.05
5.85
17.51
Standard deviation
0.13
0.37
0.68
0.12
0.34
0.62
C V (%)
6.74
5.95
4.07
5.85
5.81
3.54
Sample type &Sample volume:
serum, plasma and other biological fluids; 100μL
Reproducibility:
Both intra-CV and inter-CV are < 10%.
Application:
This ELISA kit applies to the in vitro quantitative determination of Human S100A8 concentrations in serum, plasma and other biological fluids.
Specificity:
This kit recognizes Human S100A8 in samples. No significant cross-reactivity or interference between Human S100A8 and analogues was observed.
