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Human S100 Calcium Binding Protein A9 (S100A9) ELISA Kit

SKU:
HUDL02591
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P06702
Sensitivity:
0.24ng/mL
Range:
0.781-50ng/mL
ELISA Type:
Sandwich
Synonyms:
MRP14,MIF,60B8AG,CFAG,NIF,CAGB,CGLB,L1AG,P14,Calgranulin B,Migration inhibitory factor-related protein 14,Calprotectin L1H subunit,Leukocyte L1 complex heavy chain
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
€649
Frequently bought together:

Description

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Human S100 Calcium Binding Protein A9 (S100A9) ELISA Kit

The Human S100 Calcium Binding Protein A9 (S100A9) ELISA Kit is specifically designed for the precise measurement of S100A9 levels in human serum, plasma, and cell culture supernatants. With its high sensitivity and specificity, this kit ensures accurate and consistent results, making it a valuable tool for various research applications.S100A9 is a key protein involved in inflammation and immune response, playing a pivotal role in various diseases including cancer, inflammatory disorders, and autoimmune conditions.

By detecting S100A9 levels, researchers can better understand the mechanisms underlying these diseases and potentially identify new therapeutic targets for treatment.Overall, the Human S100 Calcium Binding Protein A9 (S100A9) ELISA Kit provides researchers with a reliable and efficient method for studying the role of S100A9 in health and disease, advancing our understanding of its functions and potential clinical implications.

Product Name:Human S100 Calcium Binding Protein A9 (S100A9) ELISA Kit
Product Code:HUDL02591
Size:96 Assays
Target:S100A9
Synonyms:MRP14, MIF, 60B8AG, CFAG, NIF, CAGB, CGLB, L1AG, P14, Calgranulin B, Migration inhibitory factor-related protein 14, Calprotectin L1H subunit, Leukocyte L1 complex heavy chain
Detection Method:Sandwich
Range:0.781-50ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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