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Human S100 Calcium Binding Protein A8 (S100A8) ELISA Kit

SKU:
HUDL02590
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P05109
Sensitivity:
26pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
MRP8,MIF,60B8AG,CAGA,CFAG,CGLA,CP-10,L1Ag,NIF,P8,Calgranulin A,Cystic fibrosis antigen,Leukocyte L1 complex light chain,Migration inhibitory factor-related protein 8
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$719
Frequently bought together:

Description

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Human S100 Calcium Binding Protein A8 (S100A8) ELISA Kit

The Human S100 Calcium Binding Protein A8 (S100A8) ELISA Kit is specifically designed for the accurate detection of S100A8 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, ensuring precise and reproducible results for a variety of research applications.S100A8 is a key protein involved in calcium binding and is associated with inflammatory responses and immune regulation. It plays a crucial role in various diseases, including cancer, autoimmune disorders, and inflammatory conditions, making it a valuable biomarker for studying these diseases and developing therapeutic interventions.

With its reliable performance and user-friendly format, the Human S100A8 ELISA Kit is a valuable tool for researchers aiming to investigate the role of S100A8 in disease pathogenesis and to explore potential therapeutic strategies targeting this protein.

Product Name:Human S100 Calcium Binding Protein A8 (S100A8) ELISA Kit
Product Code:HUDL02590
Size:96 Assays
Target:S100A8
Synonyms:MRP8, MIF, 60B8AG, CAGA, CFAG, CGLA, CP-10, L1Ag, NIF, P8, Calgranulin A, Cystic fibrosis antigen, Leukocyte L1 complex light chain, Migration inhibitory factor-related protein 8
Detection Method:Sandwich
Range:78.1-5,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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