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Human S100 Calcium Binding Protein A4 (S100A4) ELISA Kit

SKU:
HUDL02586
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P26447
Sensitivity:
29pg/mL
Range:
78.125-5000pg/mL
ELISA Type:
Sandwich
Synonyms:
S100-A4,MTS1,18A2,42A,CAPL,P9KA,PEL98,Calcium protein,Calvasculin,Metastasin,Protein Mts1,Placental calcium-binding protein,Murine placental homolog
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
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Description

system_update_altDatasheet

Human S100 Calcium Binding Protein A4 (S100A4) ELISA Kit

The Human S100 Calcium Binding Protein A4 (S100A4) ELISA Kit is a highly accurate and reliable assay designed for the precise measurement of S100A4 levels in human samples such as serum, plasma, and cell culture supernatants. This kit is known for its exceptional sensitivity and specificity, ensuring consistent and reproducible results for various research applications.S100A4 is a calcium-binding protein that plays a crucial role in cell motility, invasion, and metastasis in various cancers.

Its expression is associated with tumor progression and poor prognosis, making it an important biomarker for cancer research and treatment development. By accurately measuring S100A4 levels, researchers can gain valuable insights into cancer biology and potentially identify new therapeutic targets.

Product Name:Human S100 Calcium Binding Protein A4 (S100A4) ELISA Kit
Product Code:HUDL02586
Size:96 Assays
Target:S100A4
Synonyms:S100-A4, MTS1, 18A2, 42A, CAPL, P9KA, PEL98, Calcium protein, Calvasculin, Metastasin, Protein Mts1, Placental calcium-binding protein, Murine placental homolog
Detection Method:Sandwich
Range:78.1-5,000pg/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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