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Human S100 Calcium Binding Protein A2 (S100A2) ELISA Kit(Protein S100-A2,Protein S-100L,S100 calcium-binding protein A2,S100L)

SKU:
HUDL02585
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P29034
Sensitivity:
0.124ng/mL
Range:
0.312-20ng/mL
ELISA Type:
Sandwich
Synonyms:
S100-A2,CAN19,S100L
Reactivity:
Human
Sample Type:
Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$779
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Description

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Human S100 Calcium Binding Protein A2 (S100A2) ELISA Kit (Protein S100-A2,Protein S-100L,S100 calcium-binding protein A2,S100L)

The Human S100 Calcium Binding Protein A2 (S100A2) ELISA Kit is specifically designed for the accurate and reliable detection of S100A2 levels in human serum, plasma, and cell culture supernatants. This kit offers high sensitivity and specificity, providing researchers with dependable and reproducible results for a variety of research applications.S100A2 is an important calcium-binding protein that plays a crucial role in various cellular processes, including cell differentiation, proliferation, and apoptosis.

It is implicated in a range of diseases, including cancer, inflammatory disorders, and neurodegenerative conditions, making it a valuable biomarker for studying these diseases and potential therapeutic interventions.By using the Human S100A2 ELISA Kit, researchers can gain valuable insights into the role of S100A2 in disease pathogenesis and progression, ultimately advancing our understanding of these conditions and paving the way for innovative treatment strategies.

Product Name:Human S100 Calcium Binding Protein A2 (S100A2) ELISA Kit(Protein S100-A2,Protein S-100L,S100 calcium-binding protein A2,S100L)
Product Code:HUDL02585
Size:96 Assays
Target:S100A2
Synonyms:S100-A2, CAN19, S100L
Detection Method:Sandwich
Range:0.312-20ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)80-10291
EDTA plasma (n=5)81-10090
Heparin plasma (n=5)80-8984
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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