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Human S100 Calcium Binding Protein A11 (S100A11) ELISA Kit

SKU:
HUDL02581
Product Type:
ELISA Kit
Size:
96 Assays
Uniprot:
P31949
Sensitivity:
0.067ng/mL
Range:
0.156-10ng/mL
ELISA Type:
Sandwich
Synonyms:
S100-A11,S100C,MLN70,Protein S100-C,Calgizzarin,Metastatic lymph node gene 70 protein,Protein S100-A11, N-terminally processed
Reactivity:
Human
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatants And Other Biological Fluids
$719
Frequently bought together:

Description

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Human S100 Calcium Binding Protein A11 (S100A11) ELISA Kit

The Human S100 Calcium Binding Protein A11 (S100A11) ELISA Kit is specifically designed for the accurate measurement of S100A11 levels in human serum, plasma, and cell culture supernatants. This kit boasts high sensitivity and specificity, ensuring dependable and consistent results for a variety of research purposes.S100A11 is a vital protein that plays a role in calcium binding and intracellular signaling pathways. It is known to influence cell growth, differentiation, and migration, making it a key player in various physiological and pathological processes.

Dysregulation of S100A11 has been implicated in conditions such as cancer, inflammatory diseases, and neurodegenerative disorders, highlighting its value as a biomarker for disease research and therapeutic development.With the Human S100A11 ELISA Kit, researchers can confidently measure S100A11 levels with precision and accuracy, facilitating advancements in scientific understanding and potential treatment strategies.

Product Name:Human S100 Calcium Binding Protein A11 (S100A11) ELISA Kit
Product Code:HUDL02581
Size:96 Assays
Target:S100A11
Synonyms:S100-A11, S100C, MLN70, Protein S100-C, Calgizzarin, Metastatic lymph node gene 70 protein, Protein S100-A11, N-terminally processed
Detection Method:Sandwich
Range:0.156-10ng/mL
Shelf Life:12 months
Note:For research use only
Recovery:Matrices listed below were spiked with certain level of recombinant the index and the recovery rates were calculated by comparing the measured value to the expected amount of the index in samples.
MatrixRecovery range (%)Average(%)
Serum (n=5)83-10091
EDTA plasma (n=5)90-9894
Heparin plasma (n=5)89-10295
Linearity:The linearity of the kit was assayed by testing samples spiked with appropriate concentration of the index and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample 1:2 1:4 1:8 1:16
Serum (n=5) 82-96% 83-98% 81-99% 93-101%
EDTA plasma (n=5) 88-101% 86-95% 90-102% 80-93%
Heparin plasma (n=5) 80-91% 82-90% 95-104% 79-95%
Intra-assay Precision:Intra-Assay: CV <10%. 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
Inter-assay Precision:Inter-Assay: CV <12%. 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
Stability:The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
StepProtocol
1. Prepare all reagents, samples and standards
2.Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3.Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4.Aspirate and wash 3 times
5.Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6.Aspirate and wash 5 times
7.Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8.Add 50µL Stop Solution. Read at 450nm immediately.

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